nkhani

Zowumitsira tsitsi zimagwiritsidwa ntchito nthawi zambiri ndipo zimayambitsa kuwononga tsitsi monga kuuma, kuuma ndi kutayika kwa tsitsi.Ndikofunika kumvetsetsa njira yabwino yowumitsa tsitsi popanda kuwononga.

Kafukufukuyu adawunikira kusintha kwa ultrastructure, morphology, chinyezi, ndi mtundu wa tsitsi pambuyo posambitsa mobwerezabwereza ndi kuyanika kutentha kosiyanasiyana.

Njira

Nthawi yowumitsa yokhazikika idagwiritsidwa ntchito kuwonetsetsa kuti tsitsi lililonse lauma kwathunthu, ndipo tsitsi lililonse linkathiridwa nthawi 30.Mpweya unkayikidwa pa chowumitsira tsitsi.Maluwawo adagawidwa m'magulu asanu otsatirawa: (a) palibe mankhwala, (b) kuyanika popanda chowumitsira (kutentha kwa chipinda, 20 ℃), (c) kuyanika ndi chowumitsira tsitsi kwa masekondi 60 pamtunda wa 15 cm.(47 ℃), (d) 30 masekondi ndi kuyanika tsitsi pa mtunda wa 10 cm (61 ℃), (e) kuyanika ndi tsitsi 5 cm (95 ℃) 15 masekondi.Kusanthula ndi kutumiza ma electron microscopy (TEM) ndi lipid TEM zidachitidwa.Zomwe zili m'madzi zidawunikidwa ndi chowunikira chinyezi cha halogen ndipo mtundu wa tsitsi udayesedwa ndi spectrophotometer.

Zotsatira

Pamene kutentha kumawonjezeka, pamwamba pa tsitsi kumawonongeka kwambiri.Palibe kuwonongeka kwa cortical komwe kunawonedwa, kutanthauza kuti tsitsi pamwamba limatha kukhala chotchinga cholepheretsa kuwonongeka kwa cortical.Ma cell membrane complex adawonongeka kokha mu gulu lomwe limawumitsa tsitsi lawo mwachilengedwe popanda kuyanika.Chinyezi chinali chochepa m'magulu onse ochiritsidwa poyerekeza ndi gulu lolamulira lopanda mankhwala.Komabe, kusiyana pakati pa maguluwa sikunali kofunikira.Kuyanika pansi pamikhalidwe yozungulira ndi 95 ℃ kumawoneka kusintha mtundu wa tsitsi, makamaka kupepuka, pambuyo pa machiritso 10 okha.

Mapeto

Ngakhale kugwiritsa ntchito chowumitsira chowumitsira kumawononga kwambiri kuposa kuyanika kwachilengedwe, kugwiritsa ntchito chowumitsira chowumitsa pamtunda wa 15 cm ndikusuntha kosalekeza sikuwononga kwambiri kuposa kuyanika tsitsi kwachilengedwe.